Ni Sepharose Excel, Cytiva, 17-3712-02, pack of 100 mL

Code: GE17-3712-02 D2-231

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

Purification of histidine-tagged pro...


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€1,977.30 EACH
€2,432.08 inc. VAT
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Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

Purification of histidine-tagged proteins using IMAC

General description

Ni Sepharose® excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).

Legal Information

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Sepharose is a registered trademark of Cytiva

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

average diameter90 µm
capacity≥10 mg binding capacity (histidine-tagged protein)(Dynamic binding capacity was tested with 0.5 mg/ml (histidine)6- tagged pure protein (Mr 43 000) in EX-CELL® 420 Insect serum-free medium (capacity at 10% breakthrough) or (histidine)6-tagged protein (Mr 28 000). Column volume was 1 ml and flow rate 1 ml/min. Binding capacity is sample-dependent.)
cleaning in place2-14(Cleaning-in-place: pH interval where the medium can be subjected to cleaning-in-place without significant change in function.)
manufacturer/tradenameCytiva 17-3712-02
matrixhighly cross-linked 6% agarose
packagingpack of 100 mL
shelf lifePlease be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
working range2-13
This product has met the following criteria to qualify for the following awards:



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